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Question: Biochemistry Question Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source. You can consult the bioinformatics exercise -Free Course Hero Question Answer.

Question Description: Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source. You can consult the bioinformatics exercise “Project 1: Databases for the Storage and ‘Mining’ of Genome Sequences” (ATTACHED). The procedure to identify the gene and the protein that it encodes is as follows:

** Look carefully at the DNA sequence provided (ATTACHED Q_10_i_dna_sequence.pdf) and identify the start site for transcription. **

Click on the DNA sequence from the start site of transcription and select all of the sequences and copy the sequence.

Go to the National Center for Biotechnology Information website http://www.ncbi.nlm.nih.gov/ and click on BLAST on the right-hand side under “Popular Resources”. BLAST is a program that will allow you to find the protein sequence for the DNA sequence (gene) you submit. Next click on blast on the left-hand column under the title “Basic Blast”.
Paste the DNA sequence into the box and click BLAST!. The search may take a few seconds and the page will keep updating until the search is completed.

When the search is complete you will have a figure showing the most homologous results or “sequences producing significant alignments” and following that, a list of what these proteins are. Your protein will be the first one on the list. You can click on the left-hand side on the accession number or sequence identifier information which will bring up more information. You should be able to find the name, function, size (number of amino acids), and source (name of the organism) for the protein.

Your answer should include the:
1. Amino acid sequence of the protein
2. Size of the protein
3. Identity of the protein
4. Function of the protein

question file

Free Course Hero Answer

Course Hero Answer & Explanation:

  • Amino Acid Sequence
MIPVILSGGS GSRLWPLSRK QFPKQFLALT GEHTLFQQTL ERLVFEGMDT           60         70         80         90        100  PIVVCNKDHR FIVNEQLANR KLECQRILME PFGRNTAPAV ALTAMMLVNE          110        120        130        140        150  GRDELMLVLP ADHVIDDQKA LQRALALATV AAERGEMVLF GVPATRPETG          160        170        180        190        200  YGYIKSTNDS LLPEGVSRVQ QFVEKPDEKR AVEFVKSGGY FWNSGMFLFR          210        220        230        240        250  ASRFLEELKK HDPDIYDTCV LTLERSEQTA DTVTLDDATF ACCPDNSIDY          260        270        280        290        300  AVMEKTQRAC VVPLSAGWSD VGCWASLWAV NDKDIHGNVS KGDVVIQDSR          310        320        330        340        350  NCMIHGNGKL VSVIGLDNIV VVETKDAMMI AHKDKVQGVK QMVSTLNDQG          360        370        380        390        400  RSETQNHCEV YRPWGSYDSV DMGGRFQVKH ISVKPGACLS LQMHHHRAEH          410        420        430        440        450  WIVVSGTAEV TCDENVFLLT ENQSTYIPIA SVHRLRNPGK IPLEIIEVQS          460        470        480   GSYLGEDDIE RFEDIYGRST PVERGVSVKT IAQ            
Size 481 aa  
 

 Identity: Pseudomonas fluorescens AlgA Function: Produces a precursor for alginate polymerization.Link to blast results: http://blast.ncbi.nlm.nih.gov/Blast.cgi#alnHdr_489182667

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